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6.5 l of loading buffer (95% formamide, 10 mM Tris, 1 mM EDTA, 0.025% Orange G) was then added to the mixture

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If you are unsure, start with the recommendations from the quick reference table earlier in this guide

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INGREDIENTS: Sodium Stearate and/or Sodium Myristate and /or Sodium Laurate and /or Sodium Cocoate, Aqua, Sodium Lactate, AOS, Titanium Dioxide, Aloe Barbadensis ( Aloe Vera) Extract, EDTA, Vitamin E, Carica Papaya ( Papaya Extract), Glutathione, Kojic dipalmitate, CI 15510, FD & C Yellow # 5, FD& C Blue #1, Fragrance